Because of the above-mentioned reasons, TMX represents a promising lipophilic model drug either for oral or parenteral administration using MEs as passive targeting drug delivery system. Therefore, an alternative protocol for oral, IM, or IV administration in breast cancer or in ER-negative tumors would be evaluated taking advantage of ME properties [15]. The aim of the present work was to design and characterize o/w MEs composed by pharmaceutically find more info accepted excipients for TMX delivery. They would be further proposed for alternative protocols of oral or parenteral administration. The biological behavior of the selected compositions for passive targeting drug delivery was also evaluated
#AZD9291 keyword# in MCF-7 human breast cancer cell line. 2. Materials and Methods 2.1. Material Phosphatidylcholine Inhibitors,research,lifescience,medical (PC, Phospholipon 90NG) was purchased from Phospholipid, Germany; Polyoxyethylene Sorbitan Monooleate (Polysorbate 80, PS 80) was from Fisher Chemicals, NJ, USA; Tamoxifen citrate was from Saporiti S.A., Buenos Aires, Argentina; ethanol was bought at J. T. Baker, USA; Capmul MCM L (glycerol monocaprylocaprate) and Captex 355 (caprylic/capric
Triglyceride) were purchased from Abitec, Columbus, USA. Estradiol Inhibitors,research,lifescience,medical was from Sigma Aldrich. St. Louis, MO, USA. Imwitor 408 (propylene glycol caprylate) and Myiglyol 840 (propylene glycol dicaprylate/dicaprate) were from Sasol, Witten, Inhibitors,research,lifescience,medical Germany. Oleic acid and Isopropyl mirystate were from Merck, Germany. Propylene-glycol and polyethylene glycol 400 were bought at BASF, NJ, USA. Labrafil M 1944 CS (oleoyl macrogolglycerides (polyoxylglycerides) and Transcutol P (diethylene
glycol monoethyl ether) were purchased from Gatefossé, France. All reagents were of analytical grade. Distilled water was obtained from a Milli-Q equipment. 2.2. Preliminary Solubility Evaluation for the Screening of Components PS 80 was selected as surfactant model because it is listed as a generally recognized as safe Inhibitors,research,lifescience,medical (GRAS) excipient. In addition, it is extensively used for different ways of administration, including the parenteral route [16], and for microemulsions’ preparation [8]. The solubility of TMX in a number of excipients was estimated. They Batimastat were Isopropyl myristate (IPM), Mygliol 840, Captex 355, Oleic acid, Imwitor 408, phosphatidylcholine (PC) and Capmul MCM L. PC is solid at room temperature, so a suspension was prepared (being 16% m/v the maximum concentration tested). These oils are widely used as no polar phases for ME formulation [17, 18]. PC has also been used for the formulation of parenteral MEs [19]. Regarding cosurfactants, five compounds were tested: Ethanol, Polyethilenglycol 400 (PEG 400), Transcutol P, Labrafil 1944 CS, and Propylenglycol (PG). All of them are included in the FDA inactive ingredients guide. To determine the drug solubility of TMX in excipients, drug in excess was added until turbidity was reached.