5, 5.0, 7.5 ��g/ml of METO was analyzed on 3 different days. Measure the solution at 296 nm (A1) and 223nm (A2). The results were reported in terms of relative standard deviation. Method precision Intraday Test solutions containing 2, 4, 6 ��g/ml then TELM and 2.5, 5.0, 7.5 ��g/ml of METO was analyzed three times on the same day. Measure the solution at 296 nm (A1) and 223 nm (A2). The results were reported in terms of relative standard deviation. Interday Test solution containing 2, 4, 6 ��g/ml TELM and 2.5, 5.0, 7.5 ��g/ml of METO was analyzed on 3 different days. Measure the solution at 296 nm (A1) and 223 nm (A2). The results were reported in terms of relative standard deviation. Specificity Specificity is a procedure to detect quantitatively the analyte in presence of component that may be expected to be present in the sample matrix.

Commonly used excipients in tablet preparation were spiked in a preweight quantity of drug and then absorbance was measured and calculation done to determine quantity of drugs. [Figure 6] Figure 6 UV spectrum showing standard mixture of TELM and METO (4:5 ��g/ml), test sample of TELM and METO (4:5 ��g/ml), and placebo. Accuracy The accuracy of the method was determined by calculating recoveries of TELM and METO by the standard addition method. Accuracy is performed at three levels 25, 50 and 75%. Known amount of standard solutions of TELM (0, 1, 2 and 3 ��g/ml) and METO (0, 1.25, 2.5 and 3.75 ��g/ml) were added to a pre-quantified test solution of TELM (4 ��g/mL) and METO (5 ��g/mL). Absorbance of solution was measured at selected wavelength for TELM and METO.

The amount of TELM and METO was calculated at each level by absorbance correction equation method and % recoveries were computed. Limit of detection and limit of quantitation Limit of detection is the lowest amount of analyte in a sample which can be detected but not necessarily quantitated as an exact value and limit of quantitation is the lowest amount of analyte in a sample which can be quantitatively determined with suitable precision and accuracy.[32] For these prepare linearity at the lowest concentration mixture containing TELM (0.16 – 0.24 ��g/ml) and METO (0.2 – 0.3 ��g/ml) and measure The absorbance at 296 nm and 223 nm.

Plot the calibration curve of absorbance vs concentration for individual wavelength and determine regression line equations for TELM and METO and find out the the limit of detection (LOD) and the limit of quantification (LOQ) were calculated using the standard deviation Entinostat of repeatability and slope (S) of the calibration of new calibration curve for LOD and LOQ. where, N = the standard deviation of the response and S = slope of the calibration curve. Analysis of TELM and METO in combined tablet Twenty tablets were weighed and the average weight was calculated. The tablet powder equivalent to 10 mg of TELM and 12.5 mg of METO were weighed and transferred to 100 ml volumetric flask.