Algae 2005,20(3):239–249.CrossRef 27. Kim GH, Klotchkova TA, West JA: From protoplasm to swarmer: regeneration of protoplasts from disintegrated cells of the multicellular marine green alga Microdictyon umbilicatum (Chlorophyta). J Phycol 2002,38(1):174–183.CrossRef 28. Klotchkova TA, Chah OK, West JA, Kim GH: Cytochemical and ultrastructural studies on protoplast formation from disintegrated cells of the marine alga Chaetomorpha aerea (Chlorophyta). Eur J Phycol 2003,38(3):205–216.CrossRef 29. Kim GH, Klochkova TA, Yoon KS, Song YS, Lee KP: Purification and characterization
of a lectin, Bryohealin, involved Fosbretabulin in vitro in the protplast formation of a marine green alga Bryopsis plumosa (Chlorophyta). J Phycol 2006,42(1):86–95.CrossRef 30. Yoon KS, Lee KP, Klochkova TA, Kim GH: Molecular characterization of the lectin, bryohealin, involved in protoplast regeneration of the marine alga Bryopsis plumosa (Chlorophyta). J Phycol 2008,44(1):103–112.CrossRef 31. Muller WE, Zahn RK, Kurelec B, Lucu C, Muller I, Uhlenbruck G: Lectin, a possible basis for symbiosis between
bacteria and sponges. J Bacteriol 1981,145(1):548–558.PubMed 32. De Hoff P, Brill L, Hirsch A: Plant lectins: the ties that bind in root symbiosis and plant defense. Mol Genet Genomics 2009,282(1):1–15.PubMedCrossRef check details Authors’ contributions JH designed the experiments, analysed the data and wrote the paper. FL maintained the algal cultures. JH and HD performed the experiments. FL, ODC and AW conceived the study and helped to draft the manuscript. All authors read and approved ID-8 the final manuscript.”
The integron includes a site-specific recombination system that integrates and expresses genes present on mobile elements called gene cassettes . The integron platform is defined by three characteristics: an integrase gene (intI) whose product encodes a site-specific integrase, IntI, an attachment site (attI) at which point DNA sequences are PF-02341066 in vivo inserted and a promoter (Pc) which expresses genes within the gene cassettes inserted at attI . Gene cassettes can be inserted into the integron as individual units but multiple events can lead to large tandem arrays. Integrons are best known for their role in the spread of antibiotic resistance genes in clinical environments . These clinical integrons harbour 1-6 gene cassettes and are often associated with mobile elements such as resistance plasmids and transposons . However, integrons are diverse genetic elements found in approximately 10% of environmental bacteria . In these bacteria, integrons are found in chromosomal locations and rarely carry antibiotic resistance gene cassettes indicating a general role in evolution. Vibrio is a genus of highly adaptable bacteria found in diverse marine-associated niches .