Additionally, as might be the case for the V fischeri

Additionally, as might be the case for the V. fischeri isolates that have identical 16S CX-5461 molecular weight rRNA gene structure and, presumably, are the same species, when, in fact, they are not. In this particular case, one of the isolates displayed a phosphorescent phenotype while the other did not. Similarly, V. cholerae isolates demonstrated that all had identical 16S rRNA gene sequence structures yet only two of the isolates produced identical IGS-prints. The third V. cholerae ATCC 14541 produced a distinctly different selleck screening library banding pattern. Of particular interest is that this ‘atypical’ V. cholerae strain was originally deposited as V.

albensis, not V. cholerae, underscoring the risks associated with speciating strains based solely on their 16S rRNA gene structure. To explore intraspecies level IGS-typic divergence for several important Vibrio species, a comprehensive study characterizing the

IGS-typic relationship within a population of 36 V. parahaemolyticus and 36 V. vulnificus isolates GSK126 price derived from different geographic locations was performed. As expected, these strains confirmed divergence of IGS-type patterns at the intraspecies level. Surprisingly, 15 different V. parahaemolyticus IGS-types, consisting of up to seven bands each, partitioned readily into five distinct clusters. This particular observation deviated significantly from an earlier study [26] proposing that V. parahaemolyticus was segregated into four clusters based solely on the four distinct IGS-patterns that were observed in their PAGE analysis. This significant difference in segregative

ability allows a more powerful and discriminatory resolution of strains at the intraspecies level. Furthermore, the previous study [26], suggests that mismatches in the L1 (Jensen) primer [21] gave rise to a different and, presumably, an incorrect banding pattern from that generated when using their own primer set, although the L1/G1 pattern they present in their representation is clearly in agreement with the pattern Cobimetinib clinical trial that would be theoretically obtained from the NCBI genomic sequence of the V. parahaemolyticus RIMD 2210683 strain. Interestingly, the L1/G1 pattern presented in the earlier Jensen et al. study is entirely consistent with that of our own work, which is not entirely surprising as the sequence of L1 (and G1) are 100% complementary to the annealing sites of all 11 V. parahaemolyticus RIMD 2210683 rDNA loci. We found in preliminary investigations of V. vulnificus that, although not to the degree of V. parahaemolyticus, the IGS-typing data also consisted of numerous (~10) unique patterns that partitioned nicely into four distinct clusters. Moreover, several of these isolates produced IGS-prints that consisted of five to six bands, significantly deviating from the pattern produced by our reference strains (V.

Comments are closed.