“
“Airway epithelial cells (AECs) provide the first line

of defense in the respiratory tract and are the main target of respiratory viruses. Here, using oligonucleotide and protein arrays, we analyze the infection of primary polarized human AEC cultures with influenza virus and respiratory syncytial virus (RSV), and we show that the JQ-EZ-05 immune response of AECs is quantitatively and qualitatively virus specific. Differentially expressed genes (DEGs) specifically induced by influenza virus and not by RSV included those encoding interferon B1 (IFN-B1), type III interferons (interleukin 28A [IL-28A], IL-28B, and IL-29), interleukins (IL-6, IL-1A, IL-1B, IL-23A, IL-17C, and IL-32), and chemokines Selleck GSK1210151A (CCL2, CCL8, and CXCL5). Lack of type I interferon or STAT1 signaling decreased the expression and secretion of cytokines and chemokines by the airway epithelium. We also observed strong basolateral polarization of the secretion of cytokines and chemokines by human and murine AECs during infection. Importantly, the antiviral response of human AECs to influenza virus or to RSV correlated with the infection signature obtained from peripheral blood mononuclear cells (PBMCs) isolated from patients with acute influenza or RSV bronchiolitis, respectively. IFI27 (also known as ISG12) was identified as a biomarker of respiratory virus infection in both AECs and PBMCs. In addition, the extent

of the transcriptional

perturbation in PBMCs correlated with the clinical disease severity. Our results demonstrate that the human airway epithelium mounts virus-specific immune responses that are likely to determine the subsequent systemic immune responses and suggest that the absence of epithelial immune mediators after RSV infection may contribute to explaining the inadequacy of systemic immunity to the virus.”
“Multifaceted evidence (family. twin, adoption, molecular genetic, geographic, and surname studies of suicide) suggests genetic risk factors for suicide. The migrant study design is also informative in this context, but underused. In particular, immigrant studies of suicide with a continental European host Tangeritin country are unavailable. The correspondence of suicide prevalence among 22 immigrant groups in Austria (1970-2006) with those of the homelands during the same period was analyzed. Immigrant and homeland suicide rates were significantly positively associated. Controls for age of suicide victim, immigrant group size, national pride, and quality of life in the homelands left the finding essentially unchanged. This correspondence of immigrant and country-of-birth suicide rates is consistent with the assumption of population differences in the prevalence of genetic risk factors for suicide, for which there is emerging evidence. (C) 2008 Elsevier Ireland Ltd. All rights reserved.