As a result, the PLA-MAA matrix maintained a tight interconnected networked structure and retarded the diffusion of MTX molecules. PLA imparted the nanoparticles with a certain degree of hydrophobicity, and its presence reduced the rate of matrix hydration by delaying the Idelalisib solubility penetration of H2O molecules. The combined hydration, relaxation, and degradation kinetics of PLA and MAA in the dissolution media resulted in prolonged MTX release for over 84 hours (Figure 10). The in vitro drug release data Inhibitors,research,lifescience,medical demonstrated that the PLA-MAA nanoparticulate system can provide

prolonged drug delivery (~80 hours) as compared to microparticles (12–25 hours) loaded with anticancer agents and prepared with different synthetic and natural polymer blends [45–47]. This prolonged rate of drug release allows the PLA-MAA system to be suitable for a nanoenclatherated

neuroerodible polymeric device Inhibitors,research,lifescience,medical wherein the nanoparticles can be assembled as a layer-by-layer process and provide programmable drug release of the loaded nanostructure as well as bioactives for therapeutic management of PCNSL. Figure 10 Release profile of MTX from an optimized PLA/MAA nanoparticle system with the highest drug incorporation efficiency. 3.7. Morphological Characterization of the PLA-MAA Inhibitors,research,lifescience,medical Nanoparticles SEM micrographs revealed the presence of nanoparticles that were pseudospherical in shape. At higher magnification, the surface morphology revealed a collapsed PLA-MAA matrix as a result of the curing process in the presence of 50% methanol (Figure 11(a)). SEM also showed polymer aggregates that were adsorbed onto a smooth surface. TEM images confirmed the formation of matrix-type nanoparticles with a partially formed core-shell structure represented as clear areas in the micrograph (Figure 11(b)). Figure 11 (a) SEM image showing the Inhibitors,research,lifescience,medical surface morphology of the optimized PLA/MAA nanoparticle formulation (x2500 magnification) and (b) TEM image of the optimized PLA/MAA nanoparticle formulation.

3.8. Assessment of the Thermal Properties of the PLA-MAA Nanoparticles The Inhibitors,research,lifescience,medical thermal stability of the PLA-MAA nanoparticles was investigated by temperature modulated DSC (TMDSC) with a temperature range of −35–230°C. With TMDSC, the effects of baseline slope and curvature for the analysed samples became reduced thereby increasing the sensitivity of the system. Overlapping events such as molecular Entinostat relaxation and glass transitions could be easily separated. With TMDSC, it was also possible to directly measure the Cp. TMDSC utilizes sinusoidal temperature modulations with constant heating and cooling rates typified by short small amplitudes that were able to unveil and distinguish important hidden, overlapping thermal events within the MTX-loaded PLA-MAA nanoparticle matrix. The theoretical Tg for PLA is recorded between 50 and 80°C while the Tm value is between 173 and 178°C [48]. MAA has a theoretical Tm value of 100°C and a Tg that ranges between 85 and 165°C [49].