In this work, we focus on (i) elucidating dynamics in root exudation of Solanum lycopersicum L. in an intercropping system due to AMF and/or Fol; (ii) its effect on Fol development
in vitro; and (iii) the testing of the root exudate compounds identified in the chromatographic analyses in terms of effects on fungal growth in in vitro assays. GC-MS analyses revealed an AMF-dependent increase in sugars and decrease in organic acids, mainly glucose and malate. In the HPLC analyses, an increase in chlorogenic acid Adriamycin mw was evident in the combined treatment of AMF and Fol, which is to our knowledge the first report about an increase in chlorogenic acid in root exudates of AM plants challenged with Fol compared
with plants inoculated with AMF only, clearly indicating changes in root exudation due to AMF and Fol. Root exudates of AMF tomato plants stimulate the germination rate of Fol, whereas the co-inoculation of AMF and Fol leads to a reduction in spore germination. In the in vitro assays, citrate and chlorogenic acid could be identified as possible candidates for the reduction in Fol germination rate in the root exudates of the AMF+Fol treatment because they proved inhibition at concentrations naturally occurring www.selleckchem.com/products/E7080.html in the rhizosphere. “
“To study the chemical composition of coffee beans from coffee cherries infected by brown eye spot, two experiments were conducted with coffee cherries from Catuaí Amarelo and Acaiá Cerrado farms, in the full physiological maturity stage. The coffee cherries were harvested manually, and 20 litres of cherries without
visible symptoms of brown eye spot (healthy coffee cherries) and 20 l of cherries with visible symptoms of the disease (diseased coffee cherries) were individually separated. After separation, the cherries were mixed in five different proportions to form the treatments: 0, 25, 50, 75 and 100% of diseased coffee cherries to 100, 75, 50, 25 and 0% of healthy coffee cherries. The experimental design was performed in randomized blocks, with each 8 l of coffee cherries being considered an experimental unit. After drying (humidity Fossariinae 12%), the chemical characteristics were analysed. Polyphenols, potassium leachate and electrical conductivity had a linear increase with the rising of the proportion of diseased coffee cherries. Total sugars, soluble solids and pH decreased linearly with the rising of the proportion of diseased coffee cherries. “
“Tomato (Solanum lycopersicum L.) is one of the most important vegetable crops in the world. However, the tomato production is severely affected by many diseases. The use of host resistance is believed to be the most effective approach to control the pathogens. In this study, a total of 1003 resistance-like genes were identified from the tomato genome using individual full-length search and conserved domain verification approach.