The strains of Genetic group 1 and the non-typeable strains expressed a great variety of LPS types and subtypes. All 77 Y. enterocolitica 4/O:3 and 3/O:3 strains included in the LPS analysis expressed homopolymeric subtype
GF120918 clinical trial A3 O-PS and the five Y. enterocolitica 2/O:9 strains subtype A2 O-PS (Table 3). Three of the ystB negative strains of BT 1A Genetic group 1 belonged to LPS group A2, two to C1 and one to B1c. Table 3 LPS types of 298 Y. enterocolitica BT 1A strains and 84 Y. enterocolitica strains of other biotypes LPS-type Subtype Descriptionc Commentsd Known O-serotypes with similar LPS[56] No. of strains Selleck GDC 0449 (n=382) A. Homopolymeric O-PS A1a Short O:41(27)43, O:41,43 7 A2b Medium O:10 25 A2 Medium BT 2 strains O:9 5 A3a Long 3 A3 Long BT 3–4 strains O:1, O:2, O:3 77 B. Heteropolymeric O-PS B1a a, B1b a 2/M/1 B1b strains carry homopolymer O:13,18 8 B1c a, B1d a 2+w/M/1–2 B1d strains carry homopolymer O:25 9 B2a a, B2b a 2/L/1 B2b strains carry homopolymer O:7,8, O:13,7 22 B2c a, B2d a 2+w/L/1–2 B2d strains carry homopolymer
O:50 55 B3 a 5–6+w/M/3–6 O:14, O:34, O:4,32 4 B4 a >5/M/7–10 O:4, O:8, O:21, O:35,52 1 C. Single length O-PS C1 a, SL 15-mer O:6, O:6,30, O:6,31 109 C2 a SL 30-mer O:5, O:5,27 45 D. Rough or semi-rough D a May selleck screening library include rough laboratory mutants O:15, O:28,50, O:35,36 12 a Biotype 1A, Genetic group 1. b Biotype 1A, Genetic group 2. c Homopolymeric O-PS length
estimated by migration in DOC-PAGE; heteropolymeric O-PS is described by X/Y/Z, where X = number of steps in O-PS ladder (+ w indicates a faint extra step); Y = size of step (M, medium; L, long); Z = average modality of steps; Single length O-PS migrates as one band with estimated number of sugar residues. d The biotype of the strains is 1A unless otherwise indicated. The presence of homopolymeric O-PS was visible as a smear above the short ladders and not always easy to distinguish in silver-stained DOC-PAGE gels. GNE-0877 Phage sensitivity of the strains was tested using five Yersinia specific bacteriophages (Table 4). Most of the 63 bio/serotype 3–4/O:3 strains were sensitive to ϕYeO3-12, PY100 and ϕR1-RT, in addition 7 strains were sensitive to ϕ80-81. The single bio/serotype 2/O:9 strain was infected by ϕR1-RT only. The 273 BT 1A and non-biotypeable strains representing different LPS-types showed variable phage sensitivity patterns further demonstrating the heterogeneity of this group of strains. However, all 17 of the BT 1A Genetic subgroup 2 strains were resistant to all the tested phages. Table 4 Phage sensitivities of 273 Y. enterocolitica BT 1A strains and 64 Y.