Thus, we predict that the role of repeated cocaine exposure would have differing effects from the present findings if presented prior to training.

A series of work has now suggested that repeated cocaine exposure prior to learning can result in profound deficits in acquisition. For example, cocaine-treated click here rats have been shown to have impairments in acquiring normal Pavlovian (Schoenbaum & Setlow, 2005; Saddoris et al., 2010) and operant task (Schoenbaum et al., 2004; Calu et al., 2007; Roesch et al., 2007) performance. If animals are unable to learn about cue–outcome or response–outcome associations normally as a result of cocaine exposure (a putatively core-dependent process), then such cocaine exposure should result in impaired, not enhanced, PIT due to poor initial learning, but not because of poor transfer specifically. Given that both the core and shell appear to coordinate activity to produce the PIT effect, it is not known how the core and shell subregions would coordinate activity in the course of learning to produce this phenomenon. Interestingly, many facets of NAc encoding presented here mirror results previously found

PI3K inhibitor in the amygdala. For example, similar to the core, lesions of the basolateral amygdala (BLA) disrupt behavior sensitive to Pavlovian cue encoding in similar tasks (Schoenbaum et al., 1998, 2003b; Balleine et al., 2003; Pickens et al., 2003), while also causing aberrant cue encoding in distally connected regions such as the prefrontal cortex (Schoenbaum et al., 2003a) and NAc (Ambroggi et al., 2008; Jones

et al., 2010). In contrast, the central nucleus of the amygdala (CN) has been shown to be important for attention for learning (Gallagher et al., 1990; Hatfield et al., 1996; Parkinson et al., 2000b; Haney et al., 2010), but less important for detailed cue–outcome associative learning. Consequently, similar to differences between the core and shell in the NAc, BLA and CN show a similar dissociation in PIT. CN lesions abolish potentiating transfer effects, whereas BLA lesions only appear to abolish the behavioral selectivity (i.e. only pressing the CS+-associated lever) of the PIT (Blundell et al., Osimertinib order 2001; Hall et al., 2001; Holland & Gallagher, 2003; Corbit & Balleine, 2005). These core/BLA and shell/CN parallels suggest a larger system by which the amygdala and NAc coordinate activity to produce cue-modulated instrumental behavior. Indeed, BLA inputs to the NAc (Heimer et al., 1991; Brog et al., 1993) appear to be critical for supporting cue-related learning, as asymmetric lesions of the BLA and NAc block the ability for rats to use Pavlovian cues to support new learning (Setlow et al., 2002), whereas inactivation of the BLA selectively alters NAc core encoding during appetitive conditioning (Ambroggi et al.