When it is released into the blood during the interdigestive state, motilin binds to motilin receptor and promotes
the propulsion of gut contents along the gastrointestinal tract by provoking phase III interdigestive migrating contractions. It has been suggested that there are two substyles of motilin receptors: “M (muscle)” and “N (nerve)”. The aim of this study was to identify motilin receptor expression in dogs enteric nervous system. Methods: We detected motilin receptor by immunohistochemistry. Tissues of antrum, duodenum, jejunum, ileum, proximal colon, middle colon, and distal colon were extracted from six dogs. Formalin-fixed, paraffin-embedded sections, cut at a thickness of 4 μm, were deparaffinized, rehydrated selleck compound and boiled in retrieval solution. After blocking non-specific binding see more by incubation in normal horse serum, sections were incubated with primary antibody (1:200) overnight at 4°C. Next day sections were incubated with biotinylated goat anti-rabbit antibody and horse radish peroxidase conjugated avidin 30 mins respectively. Stained sections were incubated with diaminobenzine, weakly counterstained with hematoxylin, and mounted. Results: Nerve fibers among smooth muscles and neuronal cell bodies in the myenteric plexus expressing motilin receptor immunoreactivity
were observed in antrum, duodenum, jejunum, ileum, proximal MCE公司 colon and middle colon of dogs. No motilin receptor immunoreactivity was found in smooth muscle cells. The duodenum and ileum were strongly immunoreactive than other regions and motilin receptor immunoreactivity weakened gradually in the lower part of digestive tract. Conclusion: Motilin receptors were specifically localized in dogs enteric nervous system of total gastrointestinal tract
except distal colon. Sections from duodenum and ileum exhibited strong motilin receptor immunoreativity and the expression of motilin receptor weakened gradually in the lower part of digestive tract. Key Word(s): 1. motilin receptor; 2. immunohistochemistry; 3. dog; 4. enteric nervous; Presenting Author: HONGYAN Z Additional Authors: NALI MENG, SHANSHAN CHEN Corresponding Author: NALI MENG Affiliations: The First Affiliated Hospital of Zhejiang Chinese Medical University Objective: Studying the role of the RAS – p38MAPK signaling pathway in the non-steroidal anti-inflammatory drugs-induced small intestinal injury, and its mechanism. Methods: Thirty-two male, health and clean Sprague-Dawley rats, divided randomly into blank group, model group, Valsartan group, and DX600 group, with eight for each group. The rats of blank group were given 0.9 percent normal saline 1 ml/100 g by gavage every day. The rats of model group were given diclofenac sodium and referenced to the long-term of human oral dose (75 mg/d), then conver into rats oral dose7.5 mg/d by gavage every day.