1 and 8% (w/v) NaCl. The optimal pH for growth is between 7.0 and selleck chem inhibitor 8.0 with pH 5.5 being the lowest possible pH at which growth occurs. Strain TF-218T possesses oval cells 0.4-0.9 x 0.7-2.0 ��m in size (Figure 2) and is motile by means of a single polar flagellum. On marine agar circular, slightly convex, smooth, glistering, yellowish-white colonies 1.5-2.5 mm in diameter are formed . TF-218T utilizes D-glucose, glycerol, leucine, serine, acetate, citrate and succinate . Figure 2 Scanning electron micrograph of P. daeponensis DSM 23529T In addition to the findings reported in , we observed that strain DSM 23529T is able to form blue colonies on YTSS medium, as described for the closely related strain Y4I . This is probably due to the presence of genes for indigoidine biosynthesis in the genome (see below).
The utilization of carbon compounds by P. daeponensis was also determined for this study using Generation-III microplates in an OmniLog phenotyping device (BIOLOG Inc., Hayward, CA, USA). The microplates were inoculated at 28��C with a cell suspension at a cell density of 95-96% turbidity and dye IF-A. Further additives were vitamins, micronutrients and sea-salt solutions. The exported measurement data were further analyzed with the opm package for R [30,31], using its functionality for statistically estimating parameters from the respiration curves and translating them into negative, ambiguous, and positive reactions. The strain was studied in two independent biological replicates, and reactions with a different behavior between the two repetitions were regarded as ambiguous.
For P. daeponensis strain DSM 23529T, positive reactions were observed for pH 6, 1% NaCl, 4% NaCl, 8% NaCl, D-glucose, inosine, glycerol, D-aspartic Brefeldin_A acid, L-aspartic acid, L-glutamic acid, L-histidine, L-pyroglutamic acid, L-lactic acid, ��-keto-glutaric acid, D-malic acid, L-malic acid, lithium chloride, ��-hydroxy-butyric acid, ��-hydroxy-butyric acid, ��-keto-butyric acid, acetoacetic acid, propionic acid, acetic acid and sodium bromated.